ABSTRACT

OBJECTIVE

To evaluate the effectiveness of antiretroviral therapy and the associated factors according to the type of regimen used: Single Tablet Regimen or Multiple Tablet Regimen.

METHODS

Prospective cohort of 440 patients (male, 74.3%, median age, 36 years old) who initiated antiretroviral therapy between Jan/14 and Dec/15 at a referral service in Belo Horizonte. Efficacy was defined as viral suppression (viral load, VL < 50 copies/ml) and evaluated after six and twelve months of treatment. Sociodemographic, clinical and behavioral data were collected from clinical charts and from Information Systems. Multivariate analysis of overall effectiveness was performed by logistic regression.

RESULTS

Most patients initiated Multiple Tablet Regimen antiretroviral therapy (n = 255, 58%). At six months, overall viral suppression was 74.6%, being higher among patients who used Single Tablet Regimen (80.6%, p = 0.04). At twelve months, 83.2% of patients reached viral suppression, with no difference between groups (p = 0.93). Factors independently associated with viral suppression at six and twelve months varied, being negatively associated with effectiveness: VL ≥ 100,000 copies/ml, symptoms of AIDS, longer interval time between diagnosis and initiation of antiretroviral therapy, antiretroviral switching, smoking or current illicit drugs usage (p < 0.05). Factors positively associated with viral suppression included adherence to antiretroviral therapy and category of risk/exposure of men who have sex with men (p < 0.05). Reaching viral suppression at six months was the main predictor of effectiveness at one year (OR = 8.96 and p < 0.01).

CONCLUSIONS

Viral suppression was high and better results were achieved for patients who used Single Tablet Regimen regimens at six months. Clinical, behavioral, and antiretroviral therapy -related factors influence viral suppression and highlight the need for interventions to increase early diagnosis and initiation of antiretroviral therapy, patient’s adherence, and to reduce illicit drugs and cigarette smoking in this population.

Anti-HIV Agents, administration & dosage; Antiretroviral Therapy, Highly Active; Evaluation of the Efficacy-Effectiveness of Interventions; Cohort Studies

INTRODUCTION

An estimated 830,000 people were living with HIV in Brazil by the end of 2016, a prevalence of 0.4% in the general population. This prevalence is higher among sex workers, injecting drug users, men who have sex with men (MSM), and persons deprived of liberty^aa Boletim Epidemiológico de Aids e DST. Brasília (DF): Ministério da Saúde, Secretaria de Vigilância em Saúde; 2016 [cited 2017 Mar 3];5(1). Available from: http://www.aids.gov.br/pt-br/pub/2016/boletim-epidemiologico-de-aids-2016 ^,bb Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Relatório de monitoramento clínico do HIV. Brasília (DF); 2017. Available from: http://www.aids.gov.br/pt-br/pub/2017/relatorio-de-monitoramento-clinico-do-hiv . Of this total, about 498 thousand people use antiretroviral therapy (ART), a coverage rate of 60%^bb Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Relatório de monitoramento clínico do HIV. Brasília (DF); 2017. Available from: http://www.aids.gov.br/pt-br/pub/2017/relatorio-de-monitoramento-clinico-do-hiv .

Since 1996, the Brazilian Unified Health System (SUS) has offered ART as part of its care policy for people living with HIV (PLHIV)¹1. Hallal R, Ravasi G, Kuchenbecker R, Greco D, Simão M. O acesso universal ao tratamento antirretroviral no Brasil. Tempus Actas Saude Coletiva. 2010;4(2):53-66. https://doi.org/10.18569/tempus.v4i2.791
https://doi.org/10.18569/tempus.v4i2.791... . Currently, 22 antiretroviral drugs (ARV) are provided for HIV control, including the single-tablet regimen (STR) of tenofovir, lamivudine and efavirenz, listed in 2015^cc Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Protocolo clínico e diretrizes terapêuticas para manejo da infecção pelo HIV em adultos. Brasília (DF); 2013..

The STR have allowed the simplification of ART and the administration of a single tablet per day compared to multiple-tablet regimens (MTR). The benefits include greater patient preference, increased self-perceived health, greater adherence to ART, greater viral suppression, better laboratory parameters and reduction of associated costs²2. Clay PG, Nag S, Graham CM, Narayanan S. Meta-analysis of studies comparing single and multi-tablet fixed dose combination HIV treatment regimens. Medicine (Baltimore). 2015;94(42):e1677. https://doi.org/10.1097/MD.0000000000001677
https://doi.org/10.1097/MD.0000000000001... . Thus, several clinical protocols, in agreement with the recommendations of the World Health Organization, favor the use STR as initial therapy²2. Clay PG, Nag S, Graham CM, Narayanan S. Meta-analysis of studies comparing single and multi-tablet fixed dose combination HIV treatment regimens. Medicine (Baltimore). 2015;94(42):e1677. https://doi.org/10.1097/MD.0000000000001677
https://doi.org/10.1097/MD.0000000000001... ^,dd World Health Organization. Consolidated guidelines on the use of antiretroviral drugs for treating and preventing HIV infection: recommendations for a public health approach. 2.ed. Geneva: WHO; 2016 [cited 2017 Apr 20]. Available from: http://www.who.int/hiv/pub/arv/arv-2016/en/ .

The Brazilian Ministry of Health invested over R$ 1 billion in antiretroviral drugs and the treatment of sexually transmitted diseases in 2016^ee Ministério da Transparência e Controladoria-Geral da União (BR). Portal da Transparência: gastos diretos do Governo por ação: exercício de 2016. Brasília (DF); 2017 [cited 2017 Mar 3]. Available from: http://www.transparencia.gov.br/PortalComprasDiretas . Despite the high investment, studies on the effectiveness of these drugs in Brazil are scarce. Recently published studies³3. Grangeiro A, Escuder MM, Cassanote AJF, Souza RA, Kalichman AO, Veloso V, et al. The HIV-Brazil Cohort Study: design, methods and participant characteristics. PLoS One. 2014;9(5):e95673. https://doi.org/10.1371/journal.pone.0095673
https://doi.org/10.1371/journal.pone.009... ^,44. Cardoso SW, Luz PM, Velasque L, Torres T, Coelho L, Freedberg KA, et al. Effectiveness of first-line antiretroviral therapy in the IPEC cohort, Rio de Janeiro, Brazil. AIDS Res Ther. 2014;11:29. https://doi.org/10.1186/1742-6405-11-29
https://doi.org/10.1186/1742-6405-11-29... are restricted to patients who initiated ART by 2010, when STR were not available and recommendations for initial ART depended on CD4 + T lymphocytes (CD4 + TL) and viral load (VL) levels of patients, as opposed to the current recommendation to initiate treatment promptly, regardless of such parameters^cc Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Protocolo clínico e diretrizes terapêuticas para manejo da infecção pelo HIV em adultos. Brasília (DF); 2013.. Added to that is the lack of observational studies in ART-naïve patients who used specifically the STR containing tenofovir, lamivudine and efavirenz.

We aimed to evaluate the effectiveness of antiretroviral drugs in PLHIV according to the type of regimen (STR or MTR) used in the treatment and associated factors.

METHODS

A non-concurrent prospective cohort of patients who initiated ART between January 2014 and December 2015 at an HIV specialized service (SAE) that provides inpatient and outpatient care in Belo Horizonte, the major point of reference in Minas Gerais.

A total of 1,249 patients were identified in Sistema de Controle Logístico de Medicamentos (Siclom – Logistic Control System of Medicines) and in HIV and AIDS reports issued by the SAE. Of those, 440 met the inclusion criteria (Figure). The following were considered eligible: patients aged 16 or over, diagnosed with HIV, ART-naïve and with at least one outpatient healthcare visit after initiating ART. Pregnant women were excluded due to different therapy indications.

The data were collected from medical records, Siclom and Sistema de Controle de Exames Laboratoriais da Rede Nacional de Contagem de Linfócitos CD4+/CD8+ e Carga Viral (Siscel – Laboratory Examination Control System) The data collected in medical records were: sociodemographic (sex, race, age, marital status, level of education, children, employment); behavioral and lifestyle habits (use of tobacco, alcohol and illicit drugs, prior and during follow-up); clinical/laboratory [possible sources of infection, hospitalizations in the previous year and after initiating ART, initial clinical classification of patient (according to adapted criteria of the Centers for Disease Control and Prevention^ff Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Programa Nacional de DST e Aids. Critérios de definição de casos de AIDS em adultos e crianças. Brasília (DF); 2004 [cited 2017 Apr 20]. Available from: http://bvsms.saude.gov.br/bvs/publicacoes/criterios_definicao_AIDS_adultos_criancas.pdf ) and diagnoses of comorbidities and coinfections (according to criteria of the International Classification of Diseases, tenth revision^gg World Health Organization. ICD-10: international statistical classification of diseases and related health problems: tenth revision. 2.ed. Geneva: WHO; 2004 [cited 2017 Apr 20]. Available from: http://apps.who.int/iris/handle/10665/42980 )]; and drug therapy (time between diagnosis and initial ART; therapy regimen used; record of adverse events; and adherence, characterized by the absence of non-adherence notes in the medical record).

The ARV switch was collected from Siclom and defined as replacement of an initially prescribed active ingredient. The results of the CD4 + TL and VL counts performed during follow-up were collected from Siscel or, when unavailable, from the patient’s medical record.

Data collection was carried out by two trained researchers using a standardized form and typed in EpiInfo^® 3.5.4 software. Collection and typing quality was verified by recollecting and retyping 10% of medical records with Kappa statistic value, indicating perfect inter-examiner agreement (k = 0.92)⁵5. Landis JR, Koch GG. The measurement of observer agreement for categorical data. Biometrics. 1977;33(1):159-74. https://doi.org/10.2307/2529310
https://doi.org/10.2307/2529310... .

Patient follow-up lasted 12 months. The initial date of therapy was determined by the first dispensation of ART to outpatients registered with Siclom or the first dispensation of ART registered in the medical record of patients who began therapy during hospitalization.

Therapy effectiveness was defined as viral suppression (plasma VL < 50 copies/ml) six months after initial treatment^cc Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Protocolo clínico e diretrizes terapêuticas para manejo da infecção pelo HIV em adultos. Brasília (DF); 2013.. Immunological effectiveness and recovery were evaluated as secondary outcomes (increase of more than 30% of CD4 + levels^cc Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Protocolo clínico e diretrizes terapêuticas para manejo da infecção pelo HIV em adultos. Brasília (DF); 2013.) after 12 months of follow-up. A three-month tolerance was used to collect test results to reduce the amount of missing data.

Patients were compared according to initial therapy regimen: STR or MTR. Categorical variables were presented by frequency distribution and quantitative variables by central tendency (mean or median) and variability (SD: standard deviation and TA: total amplitude). The chi-square test was used to compare the groups.

The magnitude of the association between the explanatory variables and effectiveness was estimated by odds ratio with 95% confidence interval. The independent effect of the explanatory variables was evaluated by a multiple logistic regression model which included all explanatory variables that obtained p < 0.20 in the Wald test in simple regression. The goodness of fit of the multiple model was verified by the area under the Receiver Operating Characteristic curve (above 0.7). The level of significance was 5% for all analyses, performed using R software version 3.4.0.

Three scenarios were constructed to evaluate the impact of missing data on the analysis outcomes: in the first, only patients with observed data were evaluated; in the second, missing data were considered as success (VL < 50 copies/ml), and, in the third, missing data were considered as failure (VL ≥ 50 copies/ml).

The missing data were replaced using multiple imputation by chained equations (MICE package, m = 20)⁶6. Buuren S, Groothuis-Oudshoorn K. MICE: Multivariate Imputation by Chained Equations in R. J Stat Softw. 2011;45(3). https://doi.org/10.18637/jss.v045.i03
https://doi.org/10.18637/jss.v045.i03... . The percentage of missing data was up to 30% for explanatory variables, and 23.8% at six months and 22.7% at 12 months for effectiveness. Imputation consistency was assessed by Pearson correlation between the predicted values of the estimated model using only observed data and data of the imputed model (R² = 0.99 for six months and R² = 0.83 for 12 months). No differential loss was observed between the groups that reached viral suppression or not according to the explanatory variables.

This study is part of the ECOART project “Efetividade da terapia antirretroviral em pessoas vivendo com HIV, HIV/tuberculose, HIV/hanseníase ou HIV/leishmaniose visceral, acompanhados em Belo Horizonte” (Effectiveness of antiretroviral therapy in people living with HIV, HIV/tuberculosis, HIV/leprosy or HIV/visceral leishmaniasis, followed up in Belo Horizonte) approved by the Research Ethics Committee of Universidade Federal de Minas Gerais (CAAE 31192014.3.0000.5149; 2014) and Hospital Eduardo de Menezes (877,392).

RESULTS

The median follow-up time was 11 months; 22.0% of patients were hospitalized and 11 died over this period. Most patients were male (74.3%) and the main category of risk or exposure to HIV infection was MSM (32.3%), followed by heterosexual men (30.0%). Many patients entered the cohort with advanced immunosuppression, characterized by CD4 + counts below 200 cells/mm³ (32.0%) and presence of AIDS-defining signs and symptoms (45.7%), although most had no record of hospitalization due to HIV in the year prior to entering the cohort (52.7%) (Table 1).

During follow-up, 60.7% of patients recorded infectious and parasitic diseases, of whom 28.9% presented HIV complications and 18.2% infections with a predominantly sexual mode of transmission. Co-infection by tuberculosis and hepatitis B or C occurred in 10.0% and 2.3% of the population, respectively. In addition, 25.9% of patients had a record of mental or behavioral disorder, the most common being depression (n = 67, 15.2%) and anxiety (n = 22, 5.0%).

The ART was mainly initiated at outpatient level (63.6%) with regimens that combined two nucleoside/nucleotide reverse transcriptase inhibitors (NRTI) associated with a non-nucleoside reverse transcriptase inhibitor (NNRTI) (92.0%) – mainly efavirenz (90.7%). The most common drug combination was tenofovir, lamivudine and efavirenz (85.0%), followed by zidovudine, lamivudine and efavirenz (3.6%). The interval between diagnosis and initial ART was up to 60 days for approximately half of patients (52.5%), with an average of 1.1 years (SD = 2.7; TA = 21). Adherence to treatment was higher in the first six months, 83.4% of patients, versus 73.4% at 12 months. At least one incident ART-related adverse reaction was recorded for 50.9% of patients and ARV switch for 23.9%. The use of illicit drugs and tobacco reduced after initial ART (Table 1).

The distribution of patients in the STR and MTR groups was mostly similar regarding sociodemographic, clinical, ART-related and behavioral and lifestyle characteristics. However, compared to the MTR group, patients who used STR had a higher level of education, fewer were browns, had a lower prevalence of mental disorders, more recent HIV diagnosis, and lower incidence of adverse reactions to ART and ARV switch (p < 0.05) (Table 1).

Viral suppression was observed for 74.6% of patients at six months of follow-up, and this percentage was higher among patients in the STR group (p = 0.035). Viral suppression results in the other scenarios followed the same trend, although statistical significance was observed only in the success scenario (Table 2).

At 12 months of follow-up, 83.2% of the patients reached viral suppression, with no statistically significant difference between the STR and MTR groups (p = 0.929) in all proposed scenarios. In this period, 75.2% of patients reached immunological recovery, also without differences between the groups. However, the percentage of missing data for this outcome was high (53.2%), which may compromise the interpretation of results in the proposed scenarios (Table 2).

Bivariate analysis with imputed data showed different factors associated with viral suppression at six and 12 months. Sociodemographic characteristics did not influence the outcome, whereas clinical variables related to disease progression and immunosuppression at the baseline were associated with lower effectiveness: presence of AIDS-defining signs and symptoms and hospitalization records in the year prior to entry into the cohort and viral load above of 100,000 copies/ml for effectiveness at six and 12 months (p < 0.05) (Table 3).

Among ART-related characteristics, antiretroviral drug switch was negatively associated with effectiveness at six and 12 months, while adherence was positively associated with effectiveness in both periods (p < 0.05). At six months, initiating treatment during hospitalization and use of MTR were associated with a lower probability of achieving effectiveness (p < 0.05), while initiating therapy in 2015 and diagnostic time above two months increased the likelihood of achieving viral suppression (p < 0.05). The extent of viral suppression at six months was strongly associated with achieving effectiveness at 12 months of treatment (OR = 7.78 and p < 0.001) (Table 3).

Tobacco use during follow-up was negatively associated with viral suppression, and illicit drug use during follow-up reduced the chance of achieving viral suppression at 12 months (p < 0.05) (Table 3).

Clinical, ART-related, behavioral and lifestyle factors remained in the final model associated with effectiveness in the multiple analysis with imputed data. Viral load above 100,000 copies/ml (p = 0.017) and presence of AIDS-defining signs and symptoms (p = 0.014) were associated with approximately 55.0% and 70.0% less likelihood of achieving viral suppression at six months, as were ARV switch (p < 0.001) and tobacco use during follow-up (p = 0.005). Only adherence to ART increased the chance of achieving viral suppression (OR = 2.11, p = 0.029) (Table 4).

The multiple model with imputed data showed that achieving viral suppression at six months was the main predictor of effectiveness at 12 months (OR = 8.96, p < 0.001). In addition, adherence to ART at 12 months and belonging to the MSM category increased the likelihood of achieving viral suppression (p < 0.05), while use of illicit drugs during follow-up reduced that likelihood by 66.0% (Table 4).

DISCUSSION

In this Brazilian cohort study, which included only ART-naïve patients, the overall effectiveness of antiretroviral therapy was high, 74.6% at six months and 83.2% at 12 months of treatment, similar to rates in developed countries⁷7. Marconi VC, Grandits GA, Weintrob AC, Chun H, Landrum ML, Ganesan A, et al. Outcomes of highly active antiretroviral therapy in the context of universal access to healthcare: the U.S. Military HIV Natural History Study. AIDS Res Ther. 2010;7:14. https://doi.org/10.1186/1742-6405-7-14
https://doi.org/10.1186/1742-6405-7-14... ^,88. Elzi L, Erb S, Furrer H, Ledergerber B, Cavassini M, Hirschel B, et al. Choice of initial combination antiretroviral therapy in individuals with HIV infection: determinants and outcomes. Arch Intern Med. 2012;172(17):1313-21. https://doi.org/10.1001/archinternmed.2012.3216
https://doi.org/10.1001/archinternmed.20... . The single-tablet regimen with tenofovir, lamivudine and efavirenz was associated with greater viral suppression at six months of treatment compared to multiple-tablet regimens.

The characteristics of the patients included in this study are similar to the profile of PLHIV in Brazil, as published in epidemiological bulletins and other national studies³3. Grangeiro A, Escuder MM, Cassanote AJF, Souza RA, Kalichman AO, Veloso V, et al. The HIV-Brazil Cohort Study: design, methods and participant characteristics. PLoS One. 2014;9(5):e95673. https://doi.org/10.1371/journal.pone.0095673
https://doi.org/10.1371/journal.pone.009... ^,44. Cardoso SW, Luz PM, Velasque L, Torres T, Coelho L, Freedberg KA, et al. Effectiveness of first-line antiretroviral therapy in the IPEC cohort, Rio de Janeiro, Brazil. AIDS Res Ther. 2014;11:29. https://doi.org/10.1186/1742-6405-11-29
https://doi.org/10.1186/1742-6405-11-29... ^,aa Boletim Epidemiológico de Aids e DST. Brasília (DF): Ministério da Saúde, Secretaria de Vigilância em Saúde; 2016 [cited 2017 Mar 3];5(1). Available from: http://www.aids.gov.br/pt-br/pub/2016/boletim-epidemiologico-de-aids-2016 . Patients were predominantly male, sexually infected and heterosexual. Most patients initiated ART with two NRTI associated with one NNRTI, in accordance with the Clinical Protocol and Therapeutic Guidelines in force at the time^cc Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Protocolo clínico e diretrizes terapêuticas para manejo da infecção pelo HIV em adultos. Brasília (DF); 2013..

Overall effectiveness at six months agrees with the results obtained by Cardoso et al.⁴4. Cardoso SW, Luz PM, Velasque L, Torres T, Coelho L, Freedberg KA, et al. Effectiveness of first-line antiretroviral therapy in the IPEC cohort, Rio de Janeiro, Brazil. AIDS Res Ther. 2014;11:29. https://doi.org/10.1186/1742-6405-11-29
https://doi.org/10.1186/1742-6405-11-29... in a cohort of patients in Rio de Janeiro (76.9%). At 12 months of follow-up, the result (83.2%) was slightly higher than those reported in Brazilian studies carried out between 2000 and 2010 (76.1% and 77.4%)³3. Grangeiro A, Escuder MM, Cassanote AJF, Souza RA, Kalichman AO, Veloso V, et al. The HIV-Brazil Cohort Study: design, methods and participant characteristics. PLoS One. 2014;9(5):e95673. https://doi.org/10.1371/journal.pone.0095673
https://doi.org/10.1371/journal.pone.009... ^,44. Cardoso SW, Luz PM, Velasque L, Torres T, Coelho L, Freedberg KA, et al. Effectiveness of first-line antiretroviral therapy in the IPEC cohort, Rio de Janeiro, Brazil. AIDS Res Ther. 2014;11:29. https://doi.org/10.1186/1742-6405-11-29
https://doi.org/10.1186/1742-6405-11-29... , and higher than those observed in previous studies, 46.9% and 48.4% between 1997 and 2004⁹9. Grinsztejn B, Veloso VG, Pilotto JH, Campos DP, Keruly JC, Moore RD. Comparison of clinical response to initial highly active antiretroviral therapy in the patients in clinical care in the United States and Brazil. J Acquir Immune Defic Syndr. 2007;45(5):515-20. https://doi.org/10.1097/QAI.0b013e3180decb6a
https://doi.org/10.1097/QAI.0b013e3180de... ^,1010. Acurcio FA, Puig-Junoy J, Bonolo PF, Ceccato MGB, Guimarães MDC. Análisis coste-efectividad de la adhesión inicial a la terapia antirretroviral entre individuos infectados por el VIH en Belo Horizonte, Brasil. Rev Esp Salud Publica. 2006 [cited 2017 Apr 20];80(1):41-54. Available from: http://scielo.isciii.es/pdf/resp/v80n1/original1.pdf
http://scielo.isciii.es/pdf/resp/v80n1/o... . This difference may reflect the shorter interval between diagnosis and initial therapy, according to changes in initial therapy⁷7. Marconi VC, Grandits GA, Weintrob AC, Chun H, Landrum ML, Ganesan A, et al. Outcomes of highly active antiretroviral therapy in the context of universal access to healthcare: the U.S. Military HIV Natural History Study. AIDS Res Ther. 2010;7:14. https://doi.org/10.1186/1742-6405-7-14
https://doi.org/10.1186/1742-6405-7-14... ^,cc Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das Infecções Sexualmente Transmissíveis, do HIV/AIDS e das Hepatites Virais. Protocolo clínico e diretrizes terapêuticas para manejo da infecção pelo HIV em adultos. Brasília (DF); 2013.. In addition, the better performance of newer drugs and formulations, which reduce the occurrence of adverse events, provide greater dosage convenience, and require a lower adherence rate to be effective may have contributed to this difference⁴4. Cardoso SW, Luz PM, Velasque L, Torres T, Coelho L, Freedberg KA, et al. Effectiveness of first-line antiretroviral therapy in the IPEC cohort, Rio de Janeiro, Brazil. AIDS Res Ther. 2014;11:29. https://doi.org/10.1186/1742-6405-11-29
https://doi.org/10.1186/1742-6405-11-29... ^,1111. Sutton SS, Magagnoli J, Hardin JW. Odds of viral suppression by single-tablet regimens, multiple-tablet regimens, and adherence level in HIV/AIDS patients receiving antiretroviral therapy. Pharmacotherapy. 2017;37(2):204-13. https://doi.org/10.1002/phar.1889
https://doi.org/10.1002/phar.1889... .

The simplified therapy regimen with daily ingestion of tablets only once a day is associated with the higher level of adherence of patients to ART and higher levels of viral suppression when using STR or absence of difference between groups²2. Clay PG, Nag S, Graham CM, Narayanan S. Meta-analysis of studies comparing single and multi-tablet fixed dose combination HIV treatment regimens. Medicine (Baltimore). 2015;94(42):e1677. https://doi.org/10.1097/MD.0000000000001677
https://doi.org/10.1097/MD.0000000000001... . In this cohort, effectiveness results for patients using STR were similar to results published in randomized controlled trials in ART-naïve patients, which reported viral suppression rates between 80% and 88%^12–14, albeit with different regimen compositions.

These results reinforce the strategy of supplying generic drugs in STR in Brazil. Although the difference between groups was not statistically significant at 12 months of follow-up, there was a trend of better results for patients using this drug regarding the incidence of adverse events, switch of therapy regimens, adherence to treatment and immunological recovery, as well as higher viral suppression at six months of ART.

The effectiveness of antiretroviral therapy was influenced by clinical, behavioral and ART-related factors. Among the factors that predicted effectiveness at six months, initiating treatment with high viral load and AIDS-defining signs and symptoms were negatively associated with viral suppression, as reported in previous studies^8,15–17. Initiating ART regardless of CD4+ cell counts has been adopted in Brazil since 2013. Recent studies indicate a 50% reduction in the incidence of serious AIDS-related events, such as death and opportunistic diseases, among patients who initiate ART early (CD4+ count > 500 cells/mm³)¹⁸18. INSIGHT Initiate Study Group. Initiation of antiretroviral therapy in early asymptomatic HIV infection. N Engl J Med. 2015;379(9):795-807. https://doi.org/1056/NEJMoa1506816
https://doi.org/1056/NEJMoa1506816... ^,1919. TEMPRANO ANRS 12136 Study Group. A trial of early antiretrovirals and isoniazid preventive therapy in Africa. N Engl J Med. 2015;373(9):808-22. https://doi.org/10.1056/NEJMoa1507198
https://doi.org/10.1056/NEJMoa1507198... . Patients who initiated ART within a shorter interval after diagnosis had a greater chance of achieving viral suppression at 12 months in this study.

Despite knowledge of these benefits and policies introduced to increase access to diagnosis and treatment, such as expansion of testing sites and availability of quick diagnostic tests^hh Ministério da Saúde (BR), Secretaria de Vigilância em Saúde, Departamento de Vigilância, Prevenção e Controle das IST, do HIV/AIDS e das Hepatites Virais. Relatório de monitoramento clínico do HIV - 2016. Brasília (DF); 2016 [cited 2017 Apr 20]. Available from: http://www.aids.gov.br/pt-br/pub/2016/relatorio-de-monitoramento-clinico-do-hiv-2016 , most patients initiated ART with advanced immunosuppression. The same pattern was observed in Brazilian studies in previous years³3. Grangeiro A, Escuder MM, Cassanote AJF, Souza RA, Kalichman AO, Veloso V, et al. The HIV-Brazil Cohort Study: design, methods and participant characteristics. PLoS One. 2014;9(5):e95673. https://doi.org/10.1371/journal.pone.0095673
https://doi.org/10.1371/journal.pone.009... ^,2020. Fernandes JRM, Acúrcio FA, Campos LN, Guimarães MDC. Início da terapia anti-retroviral em estágio avançado de imunodeficiência entre indivíduos portadores de HIV/AIDS em Belo Horizonte, Minas Gerais, Brasil. Cad Saude Publica. 2009;25(6):1369-80. https://doi.org/10.1590/S0102-311X2009000600019
https://doi.org/10.1590/S0102-311X200900... and may be related to difficult access to healthcare services and lack of knowledge and awareness of the population about HIV risks. This results in late search for healthcare services and, consequently, late initiation of ART²⁰20. Fernandes JRM, Acúrcio FA, Campos LN, Guimarães MDC. Início da terapia anti-retroviral em estágio avançado de imunodeficiência entre indivíduos portadores de HIV/AIDS em Belo Horizonte, Minas Gerais, Brasil. Cad Saude Publica. 2009;25(6):1369-80. https://doi.org/10.1590/S0102-311X2009000600019
https://doi.org/10.1590/S0102-311X200900... .

The MSM were more likely to achieve viral suppression after 12 months of treatment compared to other risk or exposure categories. This result may reflect a greater involvement of those patients in continuous healthcare²¹21. Burchell AN, Gardner S, Light L, Ellis BM, Antoniou T, Bacon J, et al. Implementation and operational research: engagement in HIV care among persons enrolled in a clinical HIV cohort in Ontario, Canada, 2001-2011. J Acquir Immune Defic Syndr. 2015;70(1):e10-19. https://doi.org/10.1097/QAI.0000000000000690
https://doi.org/10.1097/QAI.000000000000... . However, further studies are needed for inferences in this population.

The ARV switch and ART adherence influenced viral suppression. The ARV switch is associated with lower adherence to treatment and may be more closely related to the occurrence of adverse events and intolerance rather than virologic failure²²22. Bonolo PF, César CC, Acúrcio FA, Ceccato MGB, Pádua CAM, Álvares J, et al. Non-adherence among patients initiating antiretroviral therapy: a challenge for health professionals in Brazil. AIDS. 2005;19 Suppl 4:S5-13. https://doi.org/10.1097/01.aids.0000191484.84661.2b
https://doi.org/10.1097/01.aids.00001914... ^,2323. Heath KV, Singer J, O’Shaughnessy MV, Montaner JSG, Hogg RS. Intentional nonadherence due to adverse symptoms associated with antiretroviral therapy. J Acquir Immune Defic Syndr. 2002;31(2):211-7. https://doi.org/10.1097/01.QAI.0000026512.98625.08
https://doi.org/10.1097/01.QAI.000002651... . It may also be due to timely monitoring of effectiveness.

The relationship between adherence to treatment and ART effectiveness is well documented in the literature⁸8. Elzi L, Erb S, Furrer H, Ledergerber B, Cavassini M, Hirschel B, et al. Choice of initial combination antiretroviral therapy in individuals with HIV infection: determinants and outcomes. Arch Intern Med. 2012;172(17):1313-21. https://doi.org/10.1001/archinternmed.2012.3216
https://doi.org/10.1001/archinternmed.20... ^,1010. Acurcio FA, Puig-Junoy J, Bonolo PF, Ceccato MGB, Guimarães MDC. Análisis coste-efectividad de la adhesión inicial a la terapia antirretroviral entre individuos infectados por el VIH en Belo Horizonte, Brasil. Rev Esp Salud Publica. 2006 [cited 2017 Apr 20];80(1):41-54. Available from: http://scielo.isciii.es/pdf/resp/v80n1/original1.pdf
http://scielo.isciii.es/pdf/resp/v80n1/o... ^,1616. Fielding KL, Charalambous S, Stenson AL, Pemba LF, Martin DJ, Wood R, et al. Risk factors for poor virological outcome at 12 months in a workplace-based antiretroviral therapy programme in South Africa: a cohort study. BMC Infect Dis. 2008;8:93. https://doi.org/10.1186/1471-2334-8-93
https://doi.org/10.1186/1471-2334-8-93... and the lowest level of adherence required to ensure the effectiveness of antiretroviral drugs is between 80.0% and 95.0%¹¹11. Sutton SS, Magagnoli J, Hardin JW. Odds of viral suppression by single-tablet regimens, multiple-tablet regimens, and adherence level in HIV/AIDS patients receiving antiretroviral therapy. Pharmacotherapy. 2017;37(2):204-13. https://doi.org/10.1002/phar.1889
https://doi.org/10.1002/phar.1889... . In this study, non-adherence was observed in the medical records of 16.6% of patients over six months and 26.6% over 12 months. Such data are worrying, since medical records underestimate actual non-adherence figures. Non-adherence may lead to the development of viral resistance, progression of the disease, increased morbidity and mortality due to AIDS and contribute to increase patient care costs¹⁰10. Acurcio FA, Puig-Junoy J, Bonolo PF, Ceccato MGB, Guimarães MDC. Análisis coste-efectividad de la adhesión inicial a la terapia antirretroviral entre individuos infectados por el VIH en Belo Horizonte, Brasil. Rev Esp Salud Publica. 2006 [cited 2017 Apr 20];80(1):41-54. Available from: http://scielo.isciii.es/pdf/resp/v80n1/original1.pdf
http://scielo.isciii.es/pdf/resp/v80n1/o... ^,1111. Sutton SS, Magagnoli J, Hardin JW. Odds of viral suppression by single-tablet regimens, multiple-tablet regimens, and adherence level in HIV/AIDS patients receiving antiretroviral therapy. Pharmacotherapy. 2017;37(2):204-13. https://doi.org/10.1002/phar.1889
https://doi.org/10.1002/phar.1889... .

Viral suppression at six months was the main predictor of effectiveness in one year¹⁶16. Fielding KL, Charalambous S, Stenson AL, Pemba LF, Martin DJ, Wood R, et al. Risk factors for poor virological outcome at 12 months in a workplace-based antiretroviral therapy programme in South Africa: a cohort study. BMC Infect Dis. 2008;8:93. https://doi.org/10.1186/1471-2334-8-93
https://doi.org/10.1186/1471-2334-8-93... ^,1717. Powderly WG, Saag MS, Chapman S, Yu G, Quart B, Clendeninn NJ. Predictors of optimal virological response to potent antiretroviral therapy. AIDS. 1999;13(14):1873-80. https://doi.org/10.1097/00002030-199910010-00009
https://doi.org/10.1097/00002030-1999100... . No patients in this study had previously used ART. Considering the prevalence of HIV-resistant strains in Brazil (11.6%)²⁴24. Moraes Soares CMP, Vergara TRC, Brites C, Brito JDU, Grinberg G, Caseiro MM, et al. Prevalence of transmitted HIV-1 antiretroviral resistance among patients initiating antiretroviral therapy in Brazil: a surveillance study using dried blood spots. J Int AIDS Soc. 2014;17(1):19042. https://doi.org/10.7448/IAS.17.1.19042
https://doi.org/10.7448/IAS.17.1.19042... , failure of viral suppression is probably due to non-adherence, although resistance to NNRTI antiretroviral drugs has increased in Minas Gerais²⁵25. Duani H, Aleixo AW, Tupinambás U. Trends and predictors of HIV-1 acquired drug resistance in Minas Gerais, Brazil: 2002–2012. Braz J Infect Dis. 2017;21(2):148-54. https://doi.org/10.1016/j.bjid.2016.11.009
https://doi.org/10.1016/j.bjid.2016.11.0... . Checking patients’ adherence to therapy and carrying out actions to increase it are as important as timely monitoring of viral load and performance of genotyping tests to ensure adequate response to treatment.

Use of substances such as tobacco and illicit drugs during follow-up were associated with lower likelihood of achieving viral suppression. Tobacco use among PLHIV has been linked to worse clinical outcomes, such as increased viral load, reduced CD4+ cell count and increased occurrence of opportunistic infections²⁶26. Hile SJ, Feldman MB, Alexy ER, Irvine MK. Recent tobacco smoking is associated with poor HIV medical outcomes among HIV-infected individuals in New York. AIDS Behav. 2016;20(8):1722-9. https://doi.org/10.1007/s10461-015-1273-x
https://doi.org/10.1007/s10461-015-1273-... ^,2727. Ompad DC, Kingdon M, Kupprat S, Halkitis SN, Storholm ED, Halkitis PN. Smoking and HIV-related health issues among older HIV-positive gay, bisexual, and other men who have sex with men. Behav Med. 2014;40(3):99-107. https://doi.org/10.1080/08964289.2014.889067
https://doi.org/10.1080/08964289.2014.88... . The negative impact of tobacco use and its high prevalence in this population evidence the need to enhance non-smoking programs in HIV/AIDS specialized units.

The use of illicit substances among PLHIV may be related both to the actual source of infection and to diagnosis coping mechanisms²⁸28. Arnsten JH, Demas PA, Grant RW, Gourevitch MN, Farzadegan H, Howard AA, et al. Impact of active drug use on antiretroviral therapy adherence and viral suppression in hiv-infected drug users. J Gen Intern Med. 2002;17(5):377-81. https://doi.org/10.1046/j.1525-1497.2002.10644.x
https://doi.org/10.1046/j.1525-1497.2002... . The use of these substances is related to lower levels of adherence to treatment, lower viral suppression and lower levels of CD4+²⁸28. Arnsten JH, Demas PA, Grant RW, Gourevitch MN, Farzadegan H, Howard AA, et al. Impact of active drug use on antiretroviral therapy adherence and viral suppression in hiv-infected drug users. J Gen Intern Med. 2002;17(5):377-81. https://doi.org/10.1046/j.1525-1497.2002.10644.x
https://doi.org/10.1046/j.1525-1497.2002... ^,2929. Cofrancesco J Jr, Scherzer R, Tien PC, Gibert CL, Southwell H, Sidney S, et al. Illicit drug use and HIV treatment outcomes in a US cohort. AIDS. 2008;22(3):357-66. https://doi.org/10.1097/QAD.0b013e3282f3cc21
https://doi.org/10.1097/QAD.0b013e3282f3... . Worse results are also reported with previous use of illicit drugs³3. Grangeiro A, Escuder MM, Cassanote AJF, Souza RA, Kalichman AO, Veloso V, et al. The HIV-Brazil Cohort Study: design, methods and participant characteristics. PLoS One. 2014;9(5):e95673. https://doi.org/10.1371/journal.pone.0095673
https://doi.org/10.1371/journal.pone.009... ^,2929. Cofrancesco J Jr, Scherzer R, Tien PC, Gibert CL, Southwell H, Sidney S, et al. Illicit drug use and HIV treatment outcomes in a US cohort. AIDS. 2008;22(3):357-66. https://doi.org/10.1097/QAD.0b013e3282f3cc21
https://doi.org/10.1097/QAD.0b013e3282f3... ^,3030. Tucker JS, Burnam MA, Sherbourne CD, Kung FY, Gifford AL. Substance use and mental health correlates of nonadherence to antiretroviral medications in a sample of patients with human immunodeficiency virus infection. Am J Med. 2003;114(7):573-80. https://doi.org/10.1016/S0002-9343(03)00093-7
https://doi.org/10.1016/S0002-9343(03)00... . Treating chemical dependency, introducing other methods to help cope with the diagnosis and raising the health team’s awareness of the problem^28–30 are possible strategies to improve the clinical results of these patients.

This study has limitations, such as poor accuracy of measurements collected from medical records and high percentage of missing data. The strategies used to minimize their effect were the elaboration of clinical scenarios and data imputation, with no change in the trend of results. Study strengths include the high quality of collection, the broad inclusion of confounding factors, and the robustness of the final model in both follow-up periods

The incidence of viral suppression at six and 12 months in patients with no prior use of ART was high, with differences between patients using STR and MTR. Clinical, behavioral, lifestyle, and ART-related factors influenced viral suppression. They also demonstrated the need for interventions to improve diagnosis and the timely initiation of treatment, patients’ adherence to therapy, and the reduction of tobacco and illicit drugs use, so as to optimize treatment outcome and contribute to quality of life and reduction of HIV transmission.

Acknowledgments

Thanks to the staff at Hospital Eduardo de Menezes and Projeto ECOART, especially Romara Elizeu Amaro Perdigão and Bianca Magda Lopes de Freitas, for the help in data collection, and Jéssica Luiza Ferreira Ramos, for the contribution in statistical analyses.

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