ENVIRONMENTAL EXPOSURE TO TOBACCO SMOKE
Promoting smoke-free environments in Latin America: a comparison of methods to assess secondhand smoke exposure
Promoción de ambientes libres de humo en América Latina: una comparación de métodos para evaluar la exposición a humo de tabaco
Erika Avila-Tang, PhD, MHSI,II; Mark J Travers, PhD, MSIII; Ana Navas-Acien, MD, PhD.I,II,IV
IInstitute for Global Tobacco Control, Johns Hopkins Bloomberg School of Public Health. Baltimore, Maryland, USA.
IIDepartment of Epidemiology, Johns Hopkins Bloomberg School of Public Health. Baltimore, Maryland, USA.
IIIDepartment of Health Behavior, Roswell Park Cancer Institute. Buffalo, New York, USA.
IVDepartment of Environmental Health Sciences, Johns Hopkins Bloomberg School of Public Health. Maryland, USA.
Secondhand smoke (SHS) contains toxicants and carcinogens that are known to cause premature death and disease. Objectively measuring SHS exposure can support and evaluate smoke-free legislations. In Latin America, the most commonly used methods to measure SHS exposure are airborne nicotine and respirable suspended particles (PM2.5). Here we present results from studies conducted in public places and homes across Latin American countries. Airborne nicotine was detected in most locations between 2002-2006, before the implementation of 100% smoke-free legislation in Uruguay, Panama, Guatemala and other large cities within Latin America. Between 2006 and 2008, PM2.5 levels were found to be five times higher in places where smoking was present at the time of sampling compared to those without smoking. Measuring SHS exposure across Latin America has increased our understanding of the magnitude of exposure in this region and results have been used to effectively promote smoke-free legislation.
Keywords:air pollution, tobacco smoke; nicotine; particulate matter; surveillance
El humo de tabaco (HT) contiene tóxicos y carcinógenos que causan muerte prematura y enfermedades. La medición objetiva de la exposición en el ambiente a HT puede apoyar y evaluar las legislaciones que prohiben fumar. Aquí presentamos resultados de estudios realizados en lugares públicos y hogares latinoamericanos usando los métodos más comunes para esta exposición: nicotina y partículas respirables (PM2.5). Se detectó nicotina en el aire de la mayoría de los lugares muestreados entre 2002-2006, antes de la ejecución de la legislación 100% libre de humo en Uruguay, Panamá, y Guatemala. Entre 2006-2008, los niveles de PM2.5 resultaron ser cinco veces mayores en lugares donde personas fumaban comparado con lugares sin fumadores. Medir la exposición al HT en América Latina ha aumentado nuestra comprensión de la magnitud de la exposición en esta región y ha servido para promover eficazmente legislación libre de humo de tabaco.
Keywords: contaminación por humo de tabaco; nicotina; material particulado; vigilancia
Secondhand smoke (SHS), a mixture of mainstream and sidestream tobacco smoke, contains well known toxicants and carcinogens that cause premature death and disease worldwide.1 Mainstream smoke is the tobacco smoke exhaled by the smoker whereas sidestream smoke is the tobacco smoke released from the burning cigarette.1 In 2001, the Pan American Health Organization (PAHO) launched the Smoke-Free Americas initiative to mobilize action in support of smoke-free communities, workplaces and homes. The World Health Organization Framework Convention on Tobacco Control (WHO-FCTC), the United Nations first public health treaty, entered into force in February 2005. The FCTC was developed in response to the globalization of the tobacco epidemic and has been adopted by 168 countries (parties) as of March 4th, 2010, including 26 countries from the Americas. Article 8 of the WHO-FCTC and its implementation guidelines legally binds all parties to adopt and implement comprehensive smoke-free legislations in all public places and workplaces to protect all people from exposure to tobacco smoke.2,3
The WHO-FCTC indicates that smoke-free legislation should be monitored and evaluated, and objective measurement of SHS exposure can play a key role. First, such measurements quantify the levels of SHS to which people are exposed in critical locations. Second, determining SHS exposure can be used to assess health risks associated with SHS. Third, SHS levels can be used to educate policy makers and the population at large about SHS occurrence and the importance of enacting smoke-free legislations to eliminate health risks associated with SHS exposure. Finally, objective measures of SHS are excellent tools to evaluate smoke-free legislation once the law has been implemented.
In this review, we present a summary of the methods used to measure exposure to SHS in Latin America, results obtained using these methods, and potential next steps for the region.
Methods and environments for monitoring exposure to SHS
In general, a good marker of SHS exposure should be easily and accurately measured at an affordable cost, providing a valid assessment of SHS exposure as a whole.4 However, SHS is a dynamic and complex mixture of thousands of compounds in vapor and particulate phases. This has important implications for measuring SHS in the air, as it is not possible to directly measure SHS in its entirety. To facilitate the understanding of total suspended particles (TSP) dynamic behavior, Daisey5 proposed grouping TSP compounds into 5 major components according to their physicochemical properties (physical state, vapor pressure, and type of compound): 1) very volatile organic compounds (VVOCs) such as formaldehyde, 2) volatile organic compounds (VOCs) such as benzene, 3) semivolatile organic compounds (SVOCs) such as nicotine, 4) particulate matter (PM) and its organic compounds such as benzoapyrene, and 5) gas-phase inorganic compounds such as carbon monoxide.
Each of these different components will behave differently in the environment. The primary determinants of PM and VVOC are the amount of smoking and ventilation rates, with 20-30% of PM also being deposited on surfaces.6,7 Deposition is the process by which aerosol particles collect or deposit themselves on solid surfaces, decreasing the concentration of the particles in the air over time. For VOCs and SVOCs, such as nicotine, significant amounts of the compound will also sorb (adhere) onto room surfaces. This sorption will decrease the concentration of the SHS component in the air. Subsequent desorption (i.e. reemission into the environment), however, will increase the concentration of the component in the air. The amount and time-scale across which the sorption and desorption occurs is a function of the specific SHS component in question, ventilation rate, and the amount and type of surfaces (e.g. furnishings) in the room. Exposure to these compounds can hence occur hours, days, or even weeks after active smoking has stopped and these compounds are adsorbed and desorbed into the air of the room.6
Dozens of different markers of SHS in the air have been measured, including nicotine, respirable particles, 3-ethenylpyridine (3-EP), polycyclic aromatic hydrocarbons (PAHs), carbon monoxide, and acrolein. Each marker has advantages and disadvantages in terms of specificity, sensitivity, cost and ease of determination. Furthermore, the choice of marker will also depend on the specific question being addressed and the environment studied. Nevertheless, two methods have become the most commonly used for determining SHS exposure in different environments: airborne nicotine and respirable suspended particles. The two methods are compared side-by-side in Table I.
Airborne nicotine concentrations are well correlated with the number of cigarettes smoked and with respirable PM generated by the burning cigarette. They also provide reasonable estimates of exposure to the rest of tobacco components.5,7 Nicotine is a particularly attractive marker because tobacco smoke is its only source in most environments (i.e., it is specific to tobacco smoke) and the measurement methods, based on a small passive filter-badge, are accurate, precise, relatively inexpensive and easy to use.7,8,9 The quantification of SHS with airborne nicotine is generally made by passive sampling, a method that does not rely on mechanized air pumping. The sampling device is a small, lightweight, circular plastic badge containing a filter treated with sodium bisulfate (Figure 1). As air passes through a porous membrane, nicotine in the air is absorbed into the filter.8
After the sampling devices have been in place for a period of time in the location of interest (e.g. one to two weeks), they are sent to a laboratory where the nicotine collected by the filter is extracted into heptane with an internal standard, and then injected into a gas chromatograph, coupled with a nitrogen phosphorus detector and a capillary column.8,10 The lowest amount of nicotine that the laboratory method/instrument has been able to determine in a 7-day sample is around 0.001 µ/m3, although the limit of detection can vary across different laboratories. For quality control purposes, it is important to use around 10% duplicate samples and at least 10% blanks. Duplicate samples, a second monitor placed next to the main sampler, are used to determine how repeatable the laboratory analysis is. Correlation coefficients between 0.85 and 0.97 have been reported between duplicate and original samples.11,12,13 Blanks are monitors that are opened for 3-5 seconds at the sampling site, stored, and analyzed with the rest of the monitors. These blank samples are used to assess background nicotine levels trapped by the filter during shipping, storage and manipulation of the sampling devices. Final concentrations are calculated after subtraction of nicotine background levels from these blank samples. Because nicotine directly relates to tobacco smoke, there is no safe level of air nicotine and nicotine should be undetectable in all indoor environments. Protocols and analysis using this method are readily available at www.shsmonitoring.org.
PM can be divided into categories based on size. Respirable suspended particulates (RSP) refer only to particles small enough to be inhaled into the lower airways of the lungs. The maximum particle size for RSP is generally considered 3.5 or 4 microns. PM2.5 is a measure of the mass concentration of particles less than 2.5 microns in diameter. It is commonly used to assess SHS as it closely approximates the respirable fraction (RSP) and there are existing outdoor air quality standards based on PM2.5 concentrations that can be used for comparison.14,15 Virtually all PM in tobacco smoke is less than one micron in diameter, with the median particle size around 0.2 µm.16 ,17 Other properties of particles, besides mass concentration, can be measured including particle count and surface chemistry,18 although the relevance of these measures for secondhand monitoring is less clear.
In contrast to nicotine, PM is not specific to tobacco smoke and thus measurements in environments where smoking occurs must be compared to concentrations in comparable environments where smoking does not occur. In environments without smoking, sources of PM could be related to the presence of burning ovens and candles or to varying levels of outdoor pollution. Like nicotine, measured concentrations of SHS-associated particulate range about 100-fold, from 5 to 500 µ/m3, over a wide variety of indoor environments,19 although extreme levels of several thousand micrograms per cubic meter are not rare in some indoor environments (e.g. in certain bars and nightclubs). Indoor environments with smoking commonly have concentrations of PM2.5 and/or RSP in the range of 10-20 or even more times higher than the maximum allowed by the Environmental Protection Agency (EPA) concentrations for outdoor pollution.14,20
The novelty in PM monitoring is the availability of portable, user-friendly, affordable instruments capable of real-time continuous monitoring (one measurement every second to minutes).21 The continuous nature of measurement allows for examination of changes in tobacco smoke levels over time. These instruments allow multiple assessments of indoor air quality and are suited to check compliance with smoking policy rules. PM measurements can also be compared with health based national air quality standards for outdoor air.14 Moreover, the results of the measurements can be shared instantly with bartenders, patrons and customers, thus representing an educational opportunity. Real-time monitors can also compare outdoor and indoor air quality instantly, often a shocking experience for lay people who are accustomed to considering pollution mainly as an outdoor problem.21 The principal drawback of this marker is its poor specificity to SHS: in order to collect reliable information about exposure, it is sometimes useful to link PM measures with other specific markers such as nicotine.*
One commonly used device for measuring SHS-derived PM is the TSI SidePak AM510 Personal Aerosol Monitor (TSI, Inc., St. Paul, MN)(Figure 1). The SidePak uses a built-in sampling pump to draw air through the device where the PM in the air scatters the light from a laser. The amount of light scattering is correlated with the particle mass concentration or PM2.5. It is important to note that with any type of light-scattering instrument such as the SidePak, it is important to calibrate the device for the specific aerosol of interest, in this case tobacco smoke. The SidePak has been calibrated and validated for use in studies of SHS exposure.22,23,24,25,26 Protocols and a training course to measure SHS exposure using this device are readily available at www.tobaccofreeair.org.
Studies in Latin America
Public places: Between 2002 and 2004, we measured indoor air nicotine concentrations in public places in Argentina, Brazil, Chile, Costa Rica, Guatemala, Honduras, Mexico, Panama, Paraguay, Peru and Uruguay.11,13,27,28,29 In each of the countries, we included two secondary schools, a hospital, a government building, an airport (two airports in Argentina), and 10 restaurants and bars.
Homes: Between 2005 and 2006, we measured indoor air nicotine concentrations in approximately 40 homes of smokers and non-smokers in each of the following countries: Argentina, Brazil, Dominican Republic, Guatemala, Mexico, Panama, Peru, Uruguay, and Venezuela.12
Sampling methods: For both studies, the filter-badges were assembled at the Secondhand Smoke Exposure Assessment Laboratory of the Johns Hopkins Institute for Global Tobacco Control and shipped to each country in securely closed smoke-free containers. Trained, in-country investigators placed the small, unnoticeable filter-badges in locations selected to represent areas that people frequently occupy and spend time in. The filter-badges passively filtered the air trapping the nicotine for a period of 7,8,9,10,11,12,13,14days in each location. At the end of the sampling period, the filter-badges were sent back to the Johns Hopkins laboratory where nicotine was extracted to provide a time weighted average estimate of air nicotine concentrations (µg/m3) in each location using the method described above. Using this relatively simple method, we have monitored more than 1100 indoor public places (around 100 per country) and 400 homes across major cities in Latin America.
Air nicotine in Latin America - Key findings
Public places: Airborne nicotine was detected in most locations surveyed (>90%) confirming that smoking was widespread in indoor public environments across these Latin American countries between 2002-2004, before the implementation of 100% smoke-free legislation in Uruguay, Panama, Guatemala and other large cities within Latin America. Nicotine concentrations, however, ranged widely across locations and countries (Figure 2). Concentrations in hospitals, schools and city government buildings were highest in Argentina and Uruguay, followed by Chile and other countries. At the time of the study, legislation banning smoking in schools, hospitals and government buildings were in place in most countries. Our quantification of nicotine, however, revealed incomplete compliance with these laws and the need for better enforcement. The highest nicotine concentrations within all countries were found in bars and restaurants. Air nicotine concentrations were high even in non-smoking areas, showing once more that nonsmoking areas contiguous to smoking areas do not prevent SHS exposure. Most importantly, the high concentrations of SHS measured in restaurants and bars raised major concerns about the health of employees who work long hours in those environments. Our study clearly documented that comprehensive smoke-free laws were urgently needed to protect all people, including workers in the hospitality industry.
Homes: Airborne nicotine was detected in more than 85% of the homes surveyed. Non-smoking households had very low levels of airborne nicotine although nicotine was detected in nearly 60% of these homes. The median levels of air nicotine in households with smokers in these Latin American countries ranged from 0.04 µg/m3 in Panama, Dominican Republic, and Peru, to 1.19 µg/m3 in Argentina (Figure 3). A major concern was that air nicotine levels in some smoking households were as high as or even higher than air nicotine levels in restaurants or bars in some of the countries. Children, in particular young children, are at high risk of SHS exposure at home since they spend a large amount of time in their homes and because of their limited mobility.30"
In 2006 and 2007 PM2.5 was assessed in restaurants, bars, transportation areas such as airports and bus and train stations, and other types of venues including hotels, shopping malls, offices, casinos and schools. This study was conducted in 1822 locations in 32 countries around the world,31 including 385 locations in 5 Latin American countries: Argentina, Brazil, Mexico, Uruguay and Venezuela. In 2008, an additional 79 locations in Panama, and 56 locations in Argentina were added to the previous data collection. All of the sampling in Panama was conducted after Panama implemented nation-wide smoke-free air legislation in April 2008. Each location was visited for a minimum of 30 minutes with the SidePak monitor continuously recording PM2.5 concentrations. The number of people inside the venue and the number of burning cigarettes were recorded every 15 minutes during sampling. These observations were averaged over the time inside the venue to determine the average number of people on the premises and the average number of burning cigarettes. A sonic measuring device was used to measure room dimensions and hence the volume of each of venue where measurements were taken. The active smoker density was calculated by dividing the average number of burning cigarettes by the volume of the room in meters.
Particulate matter - Key findings in Latin America
Figure 4 shows an overall 5-fold increase in PM2.5 concentration in places where smoking was present at the time of sampling compared to those without smoking. The particle concentrations in the presence of smoking far exceeded limits established by the U.S. Environmental Protection Agency and the World Health Organization to protect human health. Brazil showed a smaller difference between places with and without smoking (2-fold) compared to other Latin American countries. This is due to the higher PM2.5 concentration in the non-smoking restaurants sampled in Brazil, likely because of the common practice of open-fire cooking in these restaurants. Compliance with the Panama smoke-free air legislation was extremely high with only a single burning cigarette observed indoors in the 79 locations sampled. As a result, public places in Panama had low levels of indoor particulate air pollution. Figure 5 compares 4 locations sampled in smoke-free Colon, Panama to 4 locations sampled in smoking-permitted Olavarria, Argentina. This example plot shows the change in PM2.5 concentrations minute-by-minute as the monitor moved between the outdoors and the four locations in each city. Immediate and dramatic increases in PM2.5 levels are seen in Olavarria as the monitor moves from outdoors to indoor places with smoking. In contrast, in Colon, levels stay low as the monitor moves between the outdoor and indoor smoke-free places.
Using monitoring data in support of smoke-free environments in Latin America
Dissemination. SHS exposure levels quantified in Latin America between 2002 and 2004 had immediate implications for public health professionals and for the government entities responsible for protecting the public from exposure to SHS. The initial peer-review publication of the study of air nicotine levels in public places11 received substantial media attention in Latin America. More importantly, partner organizations were actively involved in tobacco control activities in their countries. To help them disseminate the study findings, we prepared specific country reports trying to make them attractive and easy to understand. Tips to prepare policy relevant reports are provided in www.shsmonitoring.org. Reports included summary tables, figures, and conclusions highlighting the key points for each country. They also provided details on tobacco legislation at the country level and summarized the international evidence for smoke-free environments. Our target audiences were policy makers, medical and public health providers, media and the public at large.
Some successes. The air nicotine data and the multi-country approach proved to be powerful tools in support of smoke-free environments. Both air nicotine and PM2.5 data have been extensively used for media advocacy in Latin America. The air nicotine findings had a substantial media impact in Latin America, including at least Argentina, Uruguay, Chile and Guatemala and were actively used in support of smoke-free legislations in those countries. PM2.5 results also received very important media coverage in Argentina where PM2.5 monitoring has been used to demonstrate the need for smoke-free air legislation, to evaluate the positive impact of 100% smoke-free air legislation in some provinces, and to document the shortcomings of partial restrictions requiring only separate sections.32 In most countries, the air nicotine and PM2.5 results were presented to legislative bodies debating tobacco control legislation at the national and sub-national levels. In 2004, the Uruguayan government cited the air nicotine study in a decree that made all health care facilities smoke-free.11 Two years later, Uruguay was the first country in the Americas and the first low- or middle-income country in the world to enact comprehensive national smoke-free legislation that prohibited smoking in all indoor public places and workplaces, including bars and restaurants. In 2008, air nicotine data were used in the successful promotion of comprehensive smokefree legislation in Panama and Guatemala and PM2.5 data were used for promotion of sub-national laws in Argentina and in evaluation of the national law in Panama. In other countries, legislation remains incomplete: however, many cities are taking the lead and passing comprehensive smoke-free legislation in their jurisdictions.
Evaluation of smoke-free legislation in Latin America
We are currently revisiting locations from the 2002-2004 studies in Uruguay33 and Guatemala to measure air nicotine concentrations following the implementation of smoke-free legislation in these countries. Our questions are the following: Has exposure to SHS changed? Are levels of enforcement similar across different institutions? Are additional enforcement efforts needed? In Montevideo, Uruguay, air nicotine levels in public places and workplaces have decreased extraordinarily after the implementation of the comprehensive national smoke-free legislation in 2006.33 These findings, consistent with self-reported data on seeing smoking in regulated venues34 confirm that similar legislation can be enacted and implemented successfully in other countries. By objectively documenting decreases in SHS exposure from before to after implementation of comprehensive smoke-free legislation, we expect to encourage other countries in Latin America and other regions of the world to take the necessary steps to eliminate toxic tobacco smoke from indoor public places and workplaces.
Measuring air nicotine and respirable suspended particles in public places in Latin America has contributed to increase our understanding of the magnitude of exposure to SHS in Latin America and to use those data to support and promote compliance with smoke-free legislation. The measurement of nicotine in the home environment objectively revealed the critical need to implement educational measures that would protect children from SHS in their homes. Additional efforts to monitor and reduce SHS exposure in private environments, such as homes and motor vehicles, are needed in Latin America. While legislating smoke-free environments in private homes is challenging,35 ethical support for banning smoking across different environments can be obtained when the goal is to protect children's health.36 These environments could include multi-unit housing, parks and other outdoor places where children gather and spend time, as well as in motor vehicles. There is substantial legislative experience showing that it is possible to ban smoking in cars when children are present. Air nicotine levels37 and respirable suspended particles38 have been assessed in motor vehicles in some countries and could also be applied in Latin American countries to support smoke-free motor-vehicle legislation there. The source of SHS pollution is easily identifiable: the burning cigarette. Smoke-free environments, through legislation and education, are thus relatively simple and straightforward measures to eliminate tobacco smoke pollution.
Conducting these multi-country studies in Latin America was possible thanks to the collaboration of multiple institutions and investigators. Dr. Armando Peruga from the Pan American Health Organization (PAHO) and Drs. Patrick Breysse, Jonathan Samet and Heather Wipfli from the Johns Hopkins Bloomberg School of Public Health provided the necessary leadership to define the study goals, refine the study methods and protocols and identify the study coordinators and partnering institutions for the air nicotine in public places and home studies. The effective study conduction and dissemination of the findings in each country was possible thanks to the following lead country investigators: Marta Angueira (Argentina), Vera Colombo and Valeska Figueiredo (Brazil), Marisol Acuña (Chile), Katya Jimenez (Costa Rica), Sergio Diaz, Deborah Ossip-Klein and Essie Sierra (Dominican Republic), Joaquin Barnoya (Guatemala), Claudia Gomez (Honduras), Raydel Valdes and Luz Myriam Reynales (Mexico), Reina Roa (Panama), Graciela Gamarra (Paraguay), Carmen Barco (Peru), Adriana Blanco (Uruguay) and Natasha Herrera (Venezuela). The public places nicotine study was originally funded by PAHO and the Institute for Global Tobacco Control, and in recent years by a Clinical Investigator Award from the Flight Attendant Medical Research Institute (FAMRI), the FAMRI Center of Excellence Award to the Johns Hopkins Medical Institutions, and the Bloomberg Initiative to Reduce to Tobacco Use. The home nicotine study was funded by FAMRI through a Dr William Cahan Distinguished Professor award to Dr. Jonathan M. Samet. Dr. Travers' work in measuring SHS-derived particulate matter is also funded by FAMRI. The new particle monitoring data presented was made possible by lead investigators Verónica Schoj and María Elizabeth Pizarro in Argentina and Reina Roa in Panama.
Declaration of conflicts of interest
We declare that we have no conflicts of interest.
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Received on: March 22, 2010
Accepted on: April 21, 2010
Address reprint requests to: Erika Avila- Tang, PhD, MHS. Department of Epidemiology, Johns Hopkins Bloomberg School
of Public Health 627 N. Washington St. 2nd floor. Baltimore, Maryland, USA 21205